Inhibitory effect of modified citrus pectin and endosta tin on liver metastasis from colon cancer
LIU Hai ying, HUANG Zhi liang, YANG Guo hua
(D epartm ent of A bdom inal Surgery, The A ffiliated Cancer Ho sp ital of
Guangzhou M edical Co llege, Guangzhou, 510095, Ch ina)

514· Journal of Practical Oncology Vol. 23 No. 6 2008

Abstract: Objective To investigate the inh ibito ry effect of m odified citrus pectin (MCP ) com bined w ith endo statin (ES) on liver m etastasis from co lon cancer in m ouse. M ethods CT 226 co lon cancer cells w ere injected into the subcap sule of spleen in Balböc m ouse to establish a co lon cancer liver m etastasis m odel. O ne hundred and th irty2five m ouse w ere divided into 9 group s random ly: negative contro l group , po sitive contro l group , low M CP group , m edium M CP group and h igh MCP group , ES group , low M CP + ES group , m edium M CP + ES group and h igh M CP + ES group. T he concentrations of M CP in w ater w ere 0. 0, 0. 0, 0. 01, 0. 025, 0. 05, 0. 0, 0. 01, 0. 025, 0. 05 göL , respectively. T he concentration of ES w as 2 m gökg,
injected into the abdom inal cavity once every 2 days. T he liver m etastasis w as observed after 3 w eek s. T he exp ressions of galectin23, vascular endo thelial grow th facto r (V EGF ) and tum o r m icrovessel density (M VD ) in liver m etastasis w ere
  exam ined w ith tissue m icroarray technique and imm unoh istochem istry. T he concentrations of galectin23 and V EGF in serumw ere detected by enzym e linked imm uno so rbent assay (EL ISA ). Results T he percentage of liver m etastasis in each groupw as 100. 0% , 80. 0% , 73. 3% , 60. 0% , 86. 7% , 73. 3% , 73. 3% , 60. 0%. T he num ber of liver m etastases in h igh M CP group ,m edium M CP + ES group and h igh M CP + ES group w as significantly few er than that in po sitive contro l group (P < 0. 05).  T he num ber of liver m etastases in h igh M CP + ES group w as significantly few er than that in h igh M CP group (P < 0. 05).
T he m edian vo lum e of sp leen im p lanted tum o r in each group w as 1. 51 cm , 0. 93 cm , 0. 77 cm , 0. 70 cm , 1. 25 cm , 1. 15 cm ,
0. 75 cm and 0. 67 cm . T he size of sp leen im p lanted tum o r in m edium M CP group , h igh M CP group , m edium M CP+ ES group and h igh M CP + ES group w as sm aller com pared w ith po sitive contro l group (P < 0. 05). T he exp ression of galectin23 and
V EGF in liver m etastasis in each group w as no t significantly different (all P > 0. 05). T he concentration of galectin23 and V EGF in serum in po sitive contro l group and all treatm ent group s w as significantly h igher than that of negative group (P <
  0101 ). T here w as no significant difference betw een the po sitive contro l group and treatm ent group s (all P > 0. 05). T he M VD counts in liver m etastases w ere 28 47±3 22 27 47±3 22 26 92±1 47 26 91±2 41 26 43±1 47 26 50±3 09
  . . , . . , . . , . . , . . , . . , 25145±1. 96 and 24. 73±3. 09, respectively. Com pared w ith po sitive contro l group , the M VD of liver m etastasis focus in ES group , low M CP + ES group , m edium M CP + ES group and h igh M CP + ES group w as significantly low er (P < 0. 05).
Conclusion M CP alone o r com bine w ith ES can inh ibit liver m etastasis from co lon cancer and angiogenesis effectively in m ouse.
Key words: co lonic neop lasm s; liver neop lasm sösecondary; liver neop lasm södrug therapy; pectins; endo statinsö
therapeutic use; gene therapy