柑橘副产物发酵益生菌作为膳食补充剂对于牙鲆幼鱼生长性能、非特异性免疫和抗病性的效果

《鱼类疾病月刊》2013, 36, 617–628  

摘要

本实验进行了两项连续研究，评估的柑橘益生菌发酵副产品(CBF) 作为膳食补充剂对于牙鲆幼鱼生长性能、饲料利用率、非特异性免疫和抗病性的效果。实验1，牙鲆幼鱼共分为5组，分别是空白组（CON，饲料中不添加CBF），CBF-BS（饲料中添加3%枯草芽孢杆菌发酵产物），CBF-EF（饲料中添加3%屎肠球菌发酵产物），CBF-LR（饲料中添加3%鼠李糖乳杆菌发酵产物）和CBF-LP（饲料中添加3%植物乳酸杆菌发酵产物）。，十周饲喂结束进行测定。实验结果表明，各组之间生长性能和饲料利用率差异不显著，但是饲喂CBF的牙鲆幼鱼存活率显著高于空白组，且对于迟钝爱德华氏菌的抵抗性能明显提高。在实验2中，我们选择CBF-BS进行进一步分析。牙鲆幼鱼分别饲喂含添加0%，2%，4%，6%和8%的饲枯草芽孢杆菌发酵产物。6周饲喂实验结束进行测定。实验结果表明，CBF-BS对于牙鲆幼鱼生长性能影响不显著，但是可以有效提高幼鱼的非特异免疫力，幼鱼溶菌酶和髓过氧化物酶活性呈现剂量依赖性的增加。针对迟钝爱德华氏菌的连续性激发试验表明，CBF-BS能有效增加幼鱼的抗病能力。

Effects of dietary supplementation of citrus by-products fermented with a probiotic microbe on growth performance, innate immunity and disease resistance against Edwardsiella tarda in juvenile olive flounder, Paralichthys olivaceus (Temminck & Schlegel) 

B-J Lee, S-S Kim, J-W Song, D-H Oh, J-H Cha, J-B Jeong, M-S Heo, K-W Kim and K-J Lee.

 Journal of Fish Diseases, 2013, 36, 617–628 

Abstract

Two consecutive studies were conducted to evaluate the dietary supplementation of citrus by-products (CB) fermented with probiotic bacteria on growth performance, feed utilization, innate immune responses and disease resistance of juvenile olive flounder. In Experiment I, five diets were formulated to contain 0% (control) or 3% four different CB fermented with Bacillus subtilis(BS), Enterococcus faecium (EF), Lactobacillus rhamnosus(LR) and L. plantarum (LP) (designated as CON, CBF-BS, CBF-EF, CBF-LR and CBF-LP, respectively). During 10 weeks of a feeding trial, growth performance and feed efficiency were not significantly different among all the fish groups. However, fish fed CBF containing diets had significantly higher survivals than the CON group. Disease resistance of fish against Edwardsiella tarda was increased by the fermentation of CB. In Experiment II, we chose the BS as a promising probiotic and formulated five diets to contain 0%, 2%, 4%, 6% and 8% CBF-BS. Growth performance was not significantly affected by the CBF-BS supplementation during 6 weeks of a feeding trial. Innate immunity of fish was significantly enhanced by CBF-BS supplementation. Myeloperoxidase and lysozyme activities were increased in a dose-dependent manner by dietary CBF-BS inclusions. In a consecutive challenge test against E. tarda, an increased disease resistance was found by CBF-BS supplementation. These studies indicate that the fermentation process of CB with probiotic has beneficial effects on innate immunity and thereby increases disease resistance of olive flounder

against E. tarda. Bacillus subtiliscan be used as a promising probiotic microbe for by-product fermentation in fish feeds.